Practical HPLC Method Development 3rd Edition by Lloyd R. Snyder, Joseph J. Kirkland and Joseph L. Glajch brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method.
This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations. It details current knowledge on method development for high-performance liquid chromatography HPLC. This second edition offers increased focus on specialized applications for separating analytes such as biological samples and mixtures of enantiomers, and expanded material on computer-assisted method development, HPLC column technology, and biochemical and chiral applications, integrating background and practical material from Snyder and Kirkland's "Introduction to Modern Liquid Chromatography".
This book is aimed for scientists and students beginning work with HPLC as well as experienced analysts. The present book addresses each of these limitations of the first edition. All aspects of the subject are now covered, and the information provided has been supplemented with the literature that has appeared since 1987. Chapters 5 to 10 provide a reasonably complete account of how HPLC separation depends on the choice of column and other separation conditions.
Because the various parts of the present book are strongly interrelated, it is useful at this point to provide an overview of our subject matter and to point out how different chromatographers can use this book most effectively. Chapter 1 provides an introduction to how method development is carried out for any sample, and this should be read before proceeding further. Chapter 2 offers a review of the basics of HPLC separation, plus important background information that the chromatographer should know before beginning method development.
Finally, the resulting method must be evaluated to show that it consistently provides accurate and precise results and can be transferred to other laboratories and personnel. Chapter 15 on method validation, concludes the second edition, except for some useful appendices referred to in the text. The size of the present book presents a challenge to the reader in terms of locating material that is relevant to a particular sample or problem.
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